Mark Tseytlin, PhD

Research Interests

Research Interests

Development of novel bio-imaging methodologies, including theory, hardware and software.

The focus of our lab has been mostly on the development of novel electron paramagnetic resonance (EPR) imaging techniques. An alternatively used name is electron spin resonance (ESR). EPR is in many aspects similar to a better known nuclear magnetic resonance (NMR) modality. The same quantum mechanics laws govern both nuclear and electron spins. EPR is applied to solve medical and biological problems to a much less extent compared to NMR, and especially to its imaging modality magnetic resonance imaging (MRI). The major reason has been that protons (possess nuclear spin, S=1/2) in water are abundant in living organisms. Biomedical EPR requires the development of bio-compatible molecular probes that have to be injected or implanted into the biological object under investigation. Such probes have been developed worldwide. Our WVU collaborators Drs. Khramtsov, Bobko and Driesschaert are pioneers in the field of EPR probes synthesis. The major goal of our lab is to develop instrumentation and data processing methods that enable reliable extraction of functional information, e.g. oxygen concentration, within a limited experimental time. Compared to measurement of inanimate objects, animals and people cannot be held for a long time immobilized in the magnet. Imaging experiment lasts from 3 to 20 minutes. During this time, thousands of EPR spectra have to be measured to post-processed to form an image.

Students help winding wire around a coil that will be used in the EPR spectrometer.

A student is tuning EPR system before measurement.

Publications

Tseytlin M. General solution for rapid scan EPR deconvolution problem. J Magn Reson. 2020 Sep;318:106801. doi: 10.1016/j.jmr.2020.106801. Epub 2020 Aug 1. PMID: 32862080; PMCID: PMC7575242.

O'Connell RC, Tseytlin O, Bobko AA, Eubank TD, Tseytlin M. Rapid scan EPR: Automated digital resonator control for low-latency data acquisition. J Magn

Reson. 2022 Dec;345:107308. doi: 10.1016/j.jmr.2022.107308. Epub 2022 Oct 21. PMID: 36356489; PMCID: PMC10266206.

Tseytlin O, O'Connell R, Sivashankar V, Bobko AA, Tseytlin M. Rapid Scan EPR Oxygen Imaging in Photoactivated Resin Used for Stereolithographic 3D Printing. 3D Print Addit Manuf. 2021 Dec 1;8(6):358-365. doi: 10.1089/3dp.2020.0170. Epub 2021 Dec 9. PMID: 34977276; PMCID: PMC8713732

Oxana TA, A. Bobko  Mark, Tseytlin a. Rapid Scan EPR imaging as a Tool for Magnetic Field Mappin. Applied Magnetic Resonance. 2020;Accepted for publication.

Sanzhaeva U, Poncelet M, Tseytlin O, Tseytlin M, Gencheva M, Eubank TD, Khramtsov VV, Driesschaert B. Synthesis, Characterization and Application of a Highly Hydrophilic Triarylmethyl Radical for Biomedical EPR. The Journal of Organic Chemistry. 2020.

Tseytlin O, Guggilapu P, Bobko AA, AlAhmad H, Xu X, Epel B, O'Connell R, Hoblitzell EH, Eubank TD, Khramtsov VV, Driesschaert B, Kazkaz E, Tseytlin M. Modular imaging system: Rapid scan EPR at 800 MHz. J Magn Reson. 2019;305:94-103. Epub 2019/06/08. doi: 10.1016/j.jmr.2019.06.003. PubMed PMID: 31238278.

Poncelet M, Driesschaert B, Tseytlin O, Tseytlin M, Eubank TD, Khramtsov VV. Dextran-conjugated tetrathiatriarylmethyl radicals as biocompatible spin probes for EPR spectroscopy and imaging. Bioorg Med Chem Lett. 2019;29(14):1756-60. Epub 2019/05/13. doi: 10.1016/j.bmcl.2019.05.017. PubMed PMID: 31129052; PMCID: PMC6557268.

Sato-Akaba H, Tseytlin M. Development of an L-band rapid scan EPR digital console. J Magn Reson. 2019;304:42-52. Epub 2019/05/10. doi: 10.1016/j.jmr.2019.05.003. PubMed PMID: 31100585.

Tseytlin M, Stolin AV, Guggilapu P, Bobko AA, Khramtsov VV, Tseytlin O, Raylman RR. A combined positron emission tomography (PET)-electron paramagnetic resonance imaging (EPRI) system: initial evaluation of a prototype scanner. Phys Med Biol. 2018;63(10):105010. doi: 10.1088/1361-6560/aabfa1. PubMed PMID: 29676283; PMCID: PMC5979266.

Sanzhaeva U, Xu X, Guggilapu P, Tseytlin M, Khramtsov VV, Driesschaert B. Imaging of Enzyme Activity by Electron Paramagnetic Resonance: Concept and Experiment Using a Paramagnetic Substrate of Alkaline Phosphatase. Angew Chem Int Ed Engl. 2018;57(36):11701-5. doi: 10.1002/anie.201806851. PubMed PMID: 30003653.

Tseytlin O, Bobko A, Tseytlin M, editors. Anesthesia Free Pre-Clinical Rapid Scan Oximetry. 59th Annual Rocky Mountain Conference on Magnetic Resonance; 2018; Snowbird, Utah.

Epel B, Sundramoorthy SV, Krzykawska-Serda M, Maggio MC, Tseytlin M, Eaton GR, Eaton SS, Rosen GM, J PYK, Halpern HJ. "Imaging thiol redox status in murine tumors in vivo with rapid-scan electron paramagnetic resonance." J Magn Reson. 2017;276:31-6. doi: 10.1016/j.jmr.2016.12.015. PubMed PMID: 28092786; PMCID: PMC5336491.

Tseytlin M. Concept of Phase Cycling in Pulsed Magnetic Resonance Using Sinusoidal Magnetic Field Modulation. Z Phys Chem (N F). 2017;231(3):689-703. Epub 2016/11/09. doi: 10.1515/zpch-2016-0843. PubMed PMID: 28751814; PMCID: PMC5523863.

Tseytlin M. Full cycle rapid scan EPR deconvolution algorithm. J Magn Reson. 2017;281:272-8. doi: 10.1016/j.jmr.2017.06.008. PubMed PMID: 28666168; PMCID: PMC5568913.

Khramtsov VV, Bobko AA, Tseytlin M, Driesschaert B. "Exchange Phenomena in the Electron Paramagnetic Resonance Spectra of the Nitroxyl and Trityl Radicals: Multifunctional Spectroscopy and Imaging of Local Chemical Microenvironment." Anal Chem. 2017;89(9):4758-71. doi: 10.1021/acs.analchem.6b03796. PubMed PMID: 28363027.

Moser J, Lips K, Tseytlin M, Eaton GR, Eaton SS, Schnegg A. "Using rapid-scan EPR to improve the detection limit of quantitative EPR by more than one order of magnitude." J Magn Reson. 2017;281:17-25. doi: 10.1016/j.jmr.2017.04.003. PubMed PMID: 28500917.

Tseytlin M, Epel B, Sundramoorthy S, Tipikin D, Halpern HJ. "Decoupling of excitation and receive coils in pulsed magnetic resonance using sinusoidal magnetic field modulation." J Magn Reson. 2016;272:91-9. doi: 10.1016/j.jmr.2016.09.004. PubMed PMID: 27673275; PMCID: PMC5071169.

Biller JR, Mitchell DG, Tseytlin M, Elajaili H, Rinard GA, Quine RW, Eaton SS, Eaton GR. "Rapid Scan Electron Paramagnetic Resonance Opens New Avenues for Imaging Physiologically Important Parameters In Vivo." J Vis Exp. 2016(115). doi: 10.3791/54068. PubMed PMID: 27768025; PMCID: PMC5092072.

Z. Yu, M. Tseytlin, S.S. Eaton, and G. R. Eaton, "Multiharmonic Electron Paramagnetic Resonance for Extended Samples with both Narrow and Broad Lines", J. Magn. Reson., 254, pp 86-92

Elajaili HB, Biller JR, Tseitlin M, Dhimitruka I, Khramtsov VV, Eaton SS, Eaton GR. "Electron spin relaxation times and rapid scan EPR imaging of pH-sensitive amino-substituted trityl radicals". Magn Reson Chem. 2015 Apr;53(4):280-4. doi: 10.1002/mrc.4193. Epub 2014 Dec 12. PMID: 25504559

Biller JR, Tseitlin M, Mitchell DG, Yu Z, Buchanan LA, Elajaili H, Rosen GM, Kao JP, Eaton SS, Eaton GR. "Improved sensitivity for imaging spin trapped hydroxyl radical at 250 MHz." Chemphyschem. 2015 Feb 23;16(3):528-31. doi: 10.1002/cphc.201402835. Epub 2014 Dec 8. PMID: 25488257

Tseitlin M, Yu Z, Quine RW, Rinard GA, Eaton SS, Eaton GR. "Digitally generated excitation and near-baseband quadrature detection of rapid scan EPR signals". J Magn Reson. 2014 Oct 30;249C:126-134. doi: 10.1016/j.jmr.2014.10.011. [Epub ahead of print] PMID: 25462956

Yu Z, Quine RW, Rinard GA, Tseitlin M, Elajaili H, Kathirvelu V, Clouston LJ, Boratyński PJ, Rajca A, Stein R, Mchaourab H, Eaton SS, Eaton GR. "Rapid-scan EPR of immobilized nitroxides". J Magn Reson. 2014 Oct;247:67-71. doi: 10.1016/j.jmr.2014.08.008. Epub 2014 Aug 30. PMID: 25240151

Tseitlin M, Biller JR, Elajaili H, Khramtsov VV, Dhimitruka I, Eaton GR, Eaton SS. "New spectral-spatial imaging algorithm for full EPR spectra of multiline nitroxides and pH sensitive trityl radicals". J Magn Reson. 2014 Aug;245:150-5. doi: 10.1016/j.jmr.2014.05.013. Epub 2014 Jun 17. PMID: 25058914 Free PMC Article