Capabilities and Equipment

Testing Capability (RT-PCR tests)

• Single sample mode: 4000 samples tested alongside sequencing of380 positive samples, or 8,000 samples tested per week without sequencing.
• Pooled sample mode (4 samples per pool): 9500 samples per week alongside sequencing of 380 positive samples, or 19,000 samples tested per week without sequencing.

Testing capacity ELIZA (Antibody tests)

• 2500 samples per week

Equipment (Molecular Testing)

• Two Biomek I7 hybrid liquid handlers in HEPA filtrated enclosures
• Four 96 well real time thermocyclers
• One 384 well and two 96 well thermocyclers
• MiSeq and NextSeq 550 dx sequencers
• Agilent Tape station 4100
• Qbit fluorimeter
• Five PCR clean benches, two biosafety cabinets, centrifuges with biocontainment rotors and buckets, refrigerators and freezers

Available testing equipment at WVU RDL:

• One Biomek i7 Hybrid liquid handler for sample extraction and RT-PCR reaction setup. A second unit is used for SARS-Cov2 sequencing and can be made available for testing if needed.

• Four Biorad CFX96 real time thermocyclers
• Four PCR clean benchens
• One Beckman Avanti J15R centrifuge. A second unit is used for SARS-Cov2 sequencing and can be made available for testing if needed.
• BSL2 safety cabinet.
• One Tecan Evo 200 and Tecan Evo 150 liquid handler, reaction setup and plate reading.
• One Microdrop Dispenser for ELISA plate coating
• One additional ELISA plate reader